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. Author manuscript; available in PMC: 2015 Apr 17.
Published in final edited form as: FEBS Lett. 2014 Jan 17;588(8):1439–1445. doi: 10.1016/j.febslet.2014.01.002

Figure 2. Micro-patterned HeLa and neonatal ventricular myocyte cell pairs contain a defined cellular border containing N-cadherin and GJ plaques.

Figure 2

A) Cx43-EGFP transfected HeLa cell pairs at 3 and 9 hours post seeding were collected and fixed for antibody detection. F-actin is labeled by Phalloidin staining (red). N-cadherin (blue) is detected at the cell-cell border (arrowheads), which co-localizes with Cx43-EGFP (green) plaques at 9 hours post seeding. B) A fixed neonatal ventricular myocyte pair at 12 hours post seeding is shown. Phalloidin is used to resolve F-actin (red). Endogenous Cx43 (green) and N-cadherin (blue) co-localize at the cell-cell border (arrowheads). C) At 24 hours post seeding, a fixed cardiomyocyte pair contains Cx43 plaques (green) at the cellular border (arrowheads). Sarcomere organization is detected by α-Actinin labeling (red), while Phalloidin is used to resolve F-actin fibers (blue). Scale bar: 5µm.