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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Peptides. 2013 Nov 17;53:243–249. doi: 10.1016/j.peptides.2013.11.005

Table 1.

Primers used for the molecular cloning of the HzeaDHr

Experiments Primer names and sequences
Forward Reverse
Degenerate PCR DHrDF1: CNAAYTAYTAYYTNTTYWSNYTNGC
DHrDF2: CCNCARGARATGTAYWSNATHTGG		 DHr3′R1: GGTTGCCTTATTTCAAAGTACAC
DHr3′R2: ATCCATACTCTTTCTCACGTTG
5′ RACE GeneRacer™ 5′ Primer: CGACTGGAGCACGAGGACACTGA
GeneRacer™ 5′ Nested Primer: GGACACTGACATGGACTGAAGGAGTA		 DHr5′R1: GTTGAGATTTCAAACGAGTG
DHr5′R2: GAACTGCAGAGCTTGTGG
Full length confirmation DHr5′F2: CATCATGAGTCCCATCAGTG DHr3′R2: ATCCATACTCTTTCTCACGTTG