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. Author manuscript; available in PMC: 2015 Apr 17.
Published in final edited form as: FEBS Lett. 2014 Jan 20;588(8):1458–1464. doi: 10.1016/j.febslet.2014.01.010

Figure 1.

Figure 1

G38D and M163V are produced and form gap junction plaques similarly to WTCx40 in HeLa cells. A, Membrane topology of Cx40 with the locations of G38D and M163V indicated by circles. B, Immunoblot detection of WTCx40, G38D, and M163V in homogenates of transiently transfected HeLa cells. C, Immunofluorescent detection of Cx40 in transiently transfected HeLa cells shows that WTCx40, G38D, and M163V localize to appositional membranes (arrows) as well as within the cytoplasm. Bar, 10 μm.