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Overview of the efficiency of HRM-based SNP marker development in C. farreri
| Minor allele frequency ranges | No. of primers designed | No. of primer validated (% of designed) | No. of probe validated (% of designed) | No. of polymorphic loci (% of designed) |
|---|---|---|---|---|
| 0.4–0.5 | 1538 | 1056 (69%) | 761 (49%) | 571 (37%) |
| 0.3–0.4 | 1422 | 954 (67%) | 701 (49%) | 506 (36%) |
| 0.2–0.3 | 832 | 559 (67%) | 422 (51%) | 266 (32%) |
| 0.1–0.2 | 636 | 418 (66%) | 309 (49%) | 133 (21%) |
| 0–0.1 | 72 | 55 (76%) | 38 (53%) | 16 (22%) |
