Reagent	Components
Glial Media	425 ml MEM 5 ml GlutaMAX 5 ml sodium pyruvate, 100 mM 15 ml 20% glucose 25 ml FBS (final 5%) 25 ml calf serum (final 5%) 5 ml Penicillin/Streptomycin
0.1M Borate Buffer	2.48 g boric acid 3.9 g sodium tetraborate (Borax) 800 ml milliQ H2O pH with NaOH to 8.5 filter sterilize
Plating Media	425 ml MEM 5 ml GlutaMAX 5 ml sodium pyruvate, 100 mM 15 ml 20% glucose 50 ml FBS
NB27 Media	485 ml Neurobasal Media 5 ml GlutaMAX 2 ml B27 supplement (50x)
Dissection Buffer (BSS)	50 ml 10x HBSS 5 ml 1 M HEPES, pH 7.3 5 ml Penicillin/Streptomycin 440 ml H2O
N2.1 Media	425 ml MEM 5 ml GlutaMAX 5 ml sodium pyruvate, 100 mM 15 ml 20% glucose 50 ml ovalbumin, 1% in MEM 5 ml N2 supplement
2x HEPES Buffered Saline (HBS), sterile	274 mM NaCl 9.5 mM KCl 15 mM glucose 42 mM HEPES 1.4 mM Na2HPO4 Prepare batches of 3 different pH (7.05, 7.10, 7.15)
Wash Buffer (HEPES Buffered Saline)	50 ml 10x HBSS 5 ml 1 M HEPES, pH 7.3 445 ml H2O
50 mM Kynurenic acid	Dissolve 0.473 g of kynurenic acid in 1x PBS. Add drops of NaOH to get it into solution. Then use HCl to adjust pH back to 7.0