Figure 3.

NECA suppresses senescence by down regulating p53 and Rb in the primary rat HSC. Cells were cultured for 2–21 days and senescence detected using SA-β-Gal staining (A) at day-2 or (B) at day-21, and (C) the number of senescent cells quantified (^* = significant but p value is not applicable because first column is totally zero). (D) Western blot analysis of p53 and Rb level in the normal culture at the day-2 or, day-21. Similarly senescence was detected in the HSC treated (E) with vehicle DMSO, or (F) with NECA for 21 days. (G) The number of senescence cells was quantified in the cells treated with DMSO (CTL) or, with NECA for 21 days using Image-J cell count software (^* = <0.001). (H) Western blot analysis of p53 and Rb level in the cells treated with DMSO (CTL) or, with NECA for 21 days.