Figure 3.

A representative western blot of TGF-β1 profiles in AML exosomes. (A) Exosomes carry TGFβ1 pro-peptide consisting of latency-associated peptide (LAP) covalently bound to the mature TGFβ1 (50 kDa), cleaved LAP (37 kDa) and mature TGFβ1 (25 kDa). Ten micrograms of exosomal proteins were separated on SDS/PAGE gel and analyzed by western blots using anti-TGFβ1 and anti-CD81 antibodies. (B) Active TGFβ1 levels in the corresponding exosomes following their sonication/acidification. Results are from ELISA performed as described in the Section “Materials and Methods.”