Figure 1. Ablation of the posterior compartment.
Wing imaginal discs after 48 hr of induction of GFP (A, C and E, controls) or hid (B, D and F). Note that in both cases the βgal lineage label is acquired by all the posterior cells (A and B). The disc in B shows high levels of apoptosis, indicated by Caspase3 activity. (C and D) Cross-sections of wing discs perpendicular to the A/P border at the level of the wing pouch. The peripodial membrane is on top, the columnar epithelium on the bottom. Anterior compartment at the left, posterior at the right. There is a marked reduction of the size of P compartment in which hid is expressed (D). The epithelium is much thinner than in control disc (C), indicating a big reduction of cell number due to the ablation (compare the number of nuclei in the right part of C and D). (E–G) Shape of the A/P border after 48 hr of GFP (E) or hid (F) induction. In the later this border becomes wiggly and inter-digitized, a feature quantified in panel G. By allowing 72 hr of recovery this effect is partially recovered (third bar in panel G). Bars represent S.E.M., n>15 in each genotype and time point, *p<0.01, **p<0.05. See also Figure 1—figure supplement 1.
Figure 1—figure supplement 1. Genetic ablation and lineage labeling system.
