Figure 2. Clonal analysis of growth in discs in which the P compartment has been ablated.
(A–D) GFP-labeled clones only allowed growing during the ablation period. The clones were induced at the beginning of the ablation and the discs fixed at the end of it. Control disc (B) a hid-induced disc (C) and clone size quantification (D) are shown. Note the absence of major differences in clone size between control discs and hid-induced in both compartments. (E–H) Clones induced at the end of the ablation period and scored after 72 hr of recovery period at 17°C (see diagram E). Again, a control disc (F), a hid-induced disc (G), and quantification (H) are shown. Note in G that the clones in the P compartment are much bigger than those in the A compartment of the ablated discs and those of the P compartment in controls (F). Bars represent S.E.M., n>25 in each genotype and time point, *p<0.001.