Figure 5. Changes in epigenetic regulation during A/P boundary reconstruction.
Comparison of the size of transgressions after Hid treatment found in discs containing normal doses of Polycomb (Pc) or of trithorax (trx) (A, control with GFP treatment, B experimental), with those in discs containing one dose of Pc (C, control, D experimental) or of trx (E, control, F experimental). Note the significant increase in transgressions size in Pc3/+ background and the reduction in trxE2/+ background (a small transgression is indicated with an arrow in F). Panel G shows the quantification of the transgressions size in the three genotypes, each with respect to its own control. Bars represent S.E.M., n>15 in each genotype, *p<0.01, **p<0.05. See also Figure 5—figure supplements 1 and 2.
Figure 5—figure supplement 1. Alterations in the expression of Posterior sex comb (psc) and of H3K4 levels in the proximity of the A/P border after Hid administration.
(A and C). Psc expression (red, mtdTomato) in three control discs is uniform across the border (A), defined by the limit of the lacZ expression under hh control. Note in the three experimental discs (C) a lowering of Psc levels, indicated by the bracket. (B and D) Similar comparison of H3K4 levels between three control (B) and three experimental discs (D). H3K4 is increased in cells in the P compartment and in some anterior cells close to the border, indicated by brackets.
Figure 5—figure supplement 2. Engrailed protein levels are reduced in posterior cells.
Wing discs after 48 hr of GFP (A–A′, control) or hid (B–B′) induction in the dorsal compartment (apterous-Gal4 domain). A′ and B′ panels show a heat map representation of the Engrailed protein levels. The D/V border is indicated with a discontinuous line. In the hid-expressing disc (B–B′), note the reduction of En levels in the dorsal compared to the ventral compartment.