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. 2013 Dec 16;33(2):101–113. doi: 10.1002/embj.201283326

Figure 1.

Figure 1

Altered lineage-restricted progenitors and LSK populations in Pias1-/- mice.
  1. Cell numbers of T cells (CD4+ or CD8+), B cells (B220+), granulocytes/monocytes (Gr1+Mac1+), dendritic cells (CD11c+) and erythroids (Ter119+) in freshly isolated bone marrow (BM) from 8 to 12 weeks old WT (Pias1+/+) and Pias1−/− littermates were assayed by flow cytometry.
  2. Same as in (A) except that cell numbers of Lin− cells were assayed.
  3. Same as in (A) except that cell numbers of myeloid-restricted Lin−Sca1−c-Kit+ (L−S−K+) populations, common myeloid progenitors (CMP), granulocyte monocyte progenitors (GMP), megakaryocyte erythrocyte progenitors (MEP), common lymphoid progenitors (CLP), Pro-B and Pre-B cells as defined in supplementary Materials and Methods were assayed.
  4. Same as in (A) except that cell numbers of LSK, long-term hematopoietic stem cells (LT-HSC) and short-term multi-potent progenitors (ST/MPP) as defined in supplementary Materials and Methods in total BM were assayed.
  5. Same as in (A) except that cell numbers of dormant hematopoietic stem cells (d-HSCs; Lin−Sca1+c-Kit+CD150+CD48−CD34−) were assayed.

Data information: Shown in each panel is a pool of 3 independent experiments (n = 9–13). Error bars represent SEM. P-values were determined by non-paired t-test. See also Fig S1 and S2.