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. 2013 Dec 16;33(2):101–113. doi: 10.1002/embj.201283326

Figure 3.

Figure 3

Impaired long-term reconstitution capability and altered lineage differentiation of Pias1-/- HSCs.
  1. In vivo competitive reconstitution assays. Total bone marrow cells (2 × 105) from WT or Pias1−/− littermates (CD45.2+) were mixed with 2 × 105 of WT C57SJL bone marrow cells (CD45.1+) and injected into lethally irradiated WT C57SJL mice. The percentage of T cells (CD3+), B cells (B220+) and myeloid cells (Mac1+) from donor mice in peripheral blood (PBL) were assayed by flow cytometry at 5, 10 and 16 weeks post reconstitution.
  2. Same as in (A) except that bone marrow (BM) cells from the recipient mice were assayed at 20 weeks post reconstitution.
  3. Same as in (A) except that FACS-sorted LSK cells (1000) from WT or Pias1−/− littermates were used.
  4. Same as in (B) except that FACS-sorted LSK cells (1000) from WT or Pias1−/− littermates were used.
  5. Same as in (A) except that the percentage of T cells (CD3+), B cells (B220+) and myeloid cells (Mac1+) within the donor cells (CD45.2+) in peripheral blood (PBL) were presented.
  6. Same as in (E) except that FACS-sorted LSK cells (1000) from WT or Pias1−/− littermates were used.

Data information: Shown is a pool of 3 independent experiments in all panels (n = 10). Error bars represent SEM. P-values were determined by non-paired t-test. See also Fig S4.