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. 2013 Dec 16;33(2):101–113. doi: 10.1002/embj.201283326

Figure 4.

Figure 4

No defects in BM homing or niche retention in Pias1-/- mice.
  1. Homing of bone marrow (BM) cells. CFDA-SE (CFSE)-labeled total BM cells (2 × 107) from WT and Pias1−/− littermates were injected into lethally irradiated WT C57SJL mice. The percentage of CFSE+ cells in BM of the recipient mice was determined by flow cytometry 12 and 24 h post injection.
  2. Same as in (A) except that the number of CFSE+ cells was presented.
  3. Same as in (A) except that CFSE-labeled long-term hematopoietic stem cells (LT-HSC; Lin−Sca1+c-Kit+CD34−) cells (2000 cells/mouse) from WT and Pias1−/− littermates were used, and the number of CFSE+ cells in BM of the recipient mice was determined 24 h post injection.
  4. Niche retention assays. Total BM cells (4 × 107) from WT C57SJL mice (CD45.1+; n = 12) were injected into non-irradiated WT or Pias1−/− recipient mice (CD45.2+). The percentage of CD45.1+ cells in BM of the recipient mice were assayed by flow cytometry 12 weeks post injection.

Data information: Shown in each panel is a pool of 2 independent experiments (n = 9–10). Error bars represent SEM.