Skip to main content
. 2013 Dec 16;33(2):101–113. doi: 10.1002/embj.201283326

Figure 6.

Figure 6

Transcription of the lineage-affiliated genes is regulated by PIAS1.
  1. Quantitative real time polymerase chain reaction (Q-PCR) analyses were performed with total RNA from WT or Pias1−/− Lin− population. The expression of each gene in WT cells was relatively set as 1, and the results were adjusted by Hprt1.
  2. Same as in (A) except that common lymphoid progenitors (CLP) cells were used.
  3. Same as in (A) except that long-term hematopoietic stem cells (LT-HSC; Lin−Sca1+c-Kit+CD34−) cells were used.
  4. Same as in (A) except that CLP cells were used, and the expression of GATA1-downstream genes was quantified.

Data information: Shown in each panel is the average of 3–5 independent experiments. Error bars represent SEM. P-values were determined by non-paired t-test. See also Fig S5.