Table 1.
Evaluation by limiting dilution analysis of competitive long-term repopulating cells (CRU) in mice transplanted with WT (Pias1+/+) or Pias1-null (Pias1−/−) bone marrow cells
| Number of cells injected into secondary recipients | CRU evaluation of primary recipientsa |
|||
|---|---|---|---|---|
| 10 weeks post transplantation |
16 weeks post transplantation |
|||
| Pias1+/+ | Pias1−/− | Pias1+/+ | Pias1−/− | |
| 2,000,000 | 3/3 (24.5 ± 9.7) | 4/4 (4.7 ± 2.7) | 3/3 (32.5 ± 11.2) | 3/4 (3.9 ± 2.5) |
| 1,000,000 | 2/2 (29.5 ± 0.1) | 1/2 (1.2 ± 1.1) | 2/2 (29.4 ± 21.2) | 1/2 (1.0 ± 0.8) |
| 300,000 | 3/3 (8.9 ± 5.3) | 0/3 (0.5 ± 0.1) | 3/3 (16.5 ± 9.9) | 0/3 (0.2 ± 0.1) |
| 100,000 | 1/5 (0.5 ± 0.7) | 0/5 (0.3 ± 0.2) | 0/5 (0.2 ± 0.3) | 0/5 (0.3 ± 0.2) |
| CRU frequency per 105 cells (range)b | 0.52 (0.20–1.34) | 0.09 (0.04–0.21) | 0.37 (0.14–0.97) | 0.06 (0.02–0.15) |
a
Results are expressed as number of mice repopulated with donor-derived cells (CD45.2+; >1%) over total. Numbers in parentheses represent the mean % ± s.d. of peripheral blood CD45.2+ cells found in the transplanted recipients.
b
CRU frequency was calculated using Extreme Limiting Dilution Analysis (ELDA) software available at http://bioinf.wehi.edu.au/software/elda/.