Figure 2.

IQGAP1 localizes to the basolateral membrane in interphase and in mitotic cells, and its depolarization results in defective spindle positioning.

1. Cysts stained for tubulin (green), actin (red) and IQGAP1 (blue). Scale bars, 5 μm.
2. Cysts stained for IQGAP1 (green), actin (red) and β-catenin (blue). Scale bars, 5 μm.
3. Cysts were grown for 72 h, treated with 4 μM PMA for 5, 10 or 15 min, and stained for IQGAP1 (green), actin (red) and β-catenin (blue). The basolateral (arrows) and apical (arrowheads) localization of endogenous IQGAP1 is indicated. Scale bars, 5 μm.
4. Cysts stained with IQGAP1 (green), actin (red) and ToPro3 (blue). The yellow line represents the angle of the mitotic spindle. Scale bars, 5 μm.
5. The angle formed between the mitotic spindle and the apicobasal axis was measured and the mean angles were: Control = 72.19 ± 3.59°; PMA 5 min = 44.45 ± 5.33°; PMA 10 min = 39.30 ± 5.41°, PMA 15 min = 39.46 ± 5.29° (n = 3, >30 cells per experiment; values represent the mean ± s.e.m.). Error bars represent the s.e.m: *P < 0.05; **P < 0.01.