Figure 6.

EGFR mediates the localization of IQGAP1 to the basolateral membrane and it controls spindle orientation.

1. Scheme showing the constructs used, each designed as a GFP fusion protein. MDCK cells stably expressing C2A/B intraEGFR were grown for 72 h to form cysts, and then stained for actin (red) and IQGAP1 (blue). The apical localization of endogenous IQGAP1 is indicated (arrowheads).
2. The ability of the C2A/B intraEGFR stable clone to form single lumen cysts was quantified and represented as a percentage of the total number of cysts: control, 88.5 ± 0.7%; C2A/B intraEGFR, 45.96 ± 1.23% (n = 3, >50 cysts per experiment; values represent the mean ± s.d.).
3. C2A/B intraEGFR cysts were stained for tubulin (red) and the yellow line represents mitotic spindle angle.
4. The mean angle formed between the mitotic spindle and the apicobasal axis was: control = 75.14 ± 2.24°, intraEGFR = 44.90 ± 4.61° (n = 3, 40 cells per experiment; values represent mean ± s.e.m.).

Data information: Error bars represent the s.d. or s.e.m.: *P < 0.05; **P < 0.01. Scale bars, 5 μm.