Skip to main content
. 2014 Jan 14;33(2):129–145. doi: 10.1002/embj.201385946

Figure 8.

Figure 8

The distribution of NuMA but not LGN is altered in the absence of IQGAP1.
  1. Stable Tet-Off T23 cells expressing LGN-GFP were transfected with control or IQGAP1 siRNA. Doxycycline was removed from medium of stably expressing cells and they were immediately plated and grown in 3D cultures for 48 h. The cells were then fixed and stained for tubulin (red) and IQGAP1 (blue). The basolateral localization of the construct is indicated (arrows). Scale bars, 5 μm.
  2. MDCK cells were transfected with control or IQGAP1 siRNA and grown in 3D cultures. Cysts were fixed and stained for NuMA (green), tubulin (red) and IQGAP1 (blue). The basolateral (arrows) and apical (arrowheads) localization of NuMA is indicated. Scale bars, 5 μm.
  3. Stable Tet-Off T23 cells expressing Ct-LGN-myc were grown in 3D cultures for 72 h (left panel) or 48 h before doxycycline was removed from the medium for a further 24 h (right panel). The cells were then fixed, and stained for IQGAP1 (red) and ZO1 (blue). Scale bars, 5 μm.