Figure 3.

UbcH5c Phe62 is required for complex formation with OspG.

1. UbcH5c F62 (green) binds to a shallow pocket formed by OspG residues Y80, L99, P102, and F154.
2. Input: non-reducing samples of reaction mixtures prior to the addition of GST-OspG showing generation of E2˜Ub conjugates from wild-type UbcH5c, F62A-UbcH5c, and Ube2S. Pulldown: reducing SDS-PAGE shows GST-OspG only forms a high-affinity complex with wild-type UbcH5c˜Ub.

Source data are available online for this figure.