Figure 5.

Stronger depression during high-frequency stimulation at BSN^ΔEx4/5 synapses.

A  Example traces of EPSCs evoked at 100 Hz recorded from a Bsn^wt and a Bsn^ΔEx4/5 synapse illustrating the typical fast kinetics and short-term depression of BC EPSCs in the Bsn^wt and the deeper depression in Bsn^ΔEx4/5 synapses.

B, C  Short-term depression in response to (B) 20 stimuli applied at 100 Hz (n(Bsn^wt) = 38, n(Bsn^ΔEx4/5) = 46) and (C) in the presence of 1 mM kynurenic acid [n(Bsn^ΔEx4/5 + kyn.) = 8].

D, E  Stimulation applied at (D) 200 Hz [n(Bsn^wt) = 36, n(Bsn^ΔEx4/5) = 49] and (E) 333 Hz [n(Bsn^wt) = 30, n(Bsn^ΔEx4/5) = 41]. Black traces and grey traces are mean responses from individual Bsn^wt and Bsn^ΔEx4/5 BCs, respectively. Grand means ± SEM for control synapses are depicted in cyan for Bsn^wt and in red for Bsn^ΔEx4/5 synapses.

F  For estimation of the readily releasable pool size, EPSCs from trains were plotted cumulatively and a linear fit to the last ten amplitudes was extrapolated.

Data information: *P < 0.05; **P < 0.01; ***P < 0.001.