Fig. 6.

Overoxidation of peroxiredoxin 2 (Prdx2) and catalase activation in tubular cells. To confirm a peroxide-mediated redox cascade, tubular epithelial cell lysates were checked for Prdx2 and catalase expression after treatments. A: both BSA(FA) and palmitate treatment increased the oligomer/monomer ratio of Prdx2, together with a reduction and degradation of the monomer form at 24 h. The Western blot was run under nonreducing conditions. B: BSA(FA) and palmitate treatments first increased (6 h) the sulfenic acid form Prdx-SO₃, followed by a reduction (24 h; possibly because of degradation) as shown by Western blot analysis. C: both BSA(FA) and palmitate increased catalase expression as a line of defense against peroxides, in a dose-dependent manner at 6 and 24 h in tubular epithelial cells. Western blots are representative of at least 2–3 different experiments. *P < 0.05 vs. control, means ± SD.