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. 2014 Feb 21;15(4):411–418. doi: 10.1002/embr.201337898

Figure 3.

Figure 3

A functional Ci binding site is required for flam transcription.
  1. Schematic representations of the SF reporter constructs (left panel). The relative luciferase activity (Fluc/Rluc) was measured as described previously (right panel). Data are presented as means (n = 4). Error bars represent ± s.d. In the negative control construct (NC), a 159-bp fragment of a non-promoting sequence taken within the gfp gene was cloned upstream the −356 to fill the space between −515 and −356.
  2. Genomatix in silico analysis of the region from −515 to −356 upstream of the transcription start site (TSS). Boxes indicate transcription factor-binding sites. Grey boxes indicate transcription factors known to be expressed in ovarian somatic stem (OSS) cells (modEncode data).
  3. Schematic representation of firefly reporters carrying deletions of each predicted transcription factor-binding site. The Fluc/Rluc activity was measured as described previously (right panel). Data are presented as means (n = 4). Error bars represent ± s.d.