Fig. 1.

Expression of myosin II isoforms. Western blot of myosin IIA, IIB, and IIC in parietal cells. Isolated cells from rabbit stomachs were treated with either 100 μM cimetidine (Control) or 100 μM histamine (Histamine) for 30 min, then processed for Western blots to assay myosin heavy chain. Equal amounts of protein were loaded onto 4–12% SDS-PAGE gels. Proteins were transferred onto membranes and probed with antibodies to myosin heavy chain A, B, or C. Results were visualized with chemiluminescence reagent. There were no differences in myosin band intensities between control and histamine-stimulated cells.