Fig. 10.

Effects of combinations of jasplakinolide, blebbistatin, ML-7, and nocodazole on parietal cell ezrin, myosin IIB, and tubulin. Parietal cells were treated with blebbistatin (50 μM) for 30 min followed by jasplakinolide (3 μM) for another 30 min (Blebbistatin + Jasplakinolide) (A) or with ML-7 (10 μM) for 30 min followed by jasplakinolide for 30 min (ML-7 + Jasplakinolide) (B) or with nocodazole (20 μM) for 4 h followed by jasplakinolide for 30 min (Nocodazole + Jasplakinolide) (C); cells were then fixed and stained for ezrin, myosin IIB, and tubulin as described in Fig. 9.