Fig. 3.

LPA5 expression in CGRP and the transient receptor potential cation channel subfamily V member 1 (TrpV1) expressing neurons of the mouse dorsal root ganglia (DRG). A and B: LPA5-LI was predominantly detected on the surface plasma lemma of the CGRP-positive DRG neurons (D is the enlarged inset in C). E: single-cell RT-PCR analysis of DRG neurons from C57BL/6J mice. The mRNA-encoding actin, TRPV1, and LPA5 from small-diameter neurons were amplified. Results from 13 neurons (of 78 neurons from 7 mice) are shown. Neurons 1, 3, 5, 8, 9, and 12 coexpressed LPA5 and TRPV1. F: Venn diagram illustrates the proportion of small-diameter neurons expressing LPA5 and TRPV1. A 47% subset of LPA5-positive neurons coexpressed TRPV1. No transcripts were amplified from bath fluid.