Fig. 5.

Increase in c-Fos and cAMP response element (CRE) promoter activity upon LPA5 activation in differentiated PC12 cells. NGF differentiated PC12 cells were transfected with a c-Fos promoter-linked luciferase (A) or a CRE promoter-linked luciferase (B) reporter plasmid plus either the empty vector or LPA5 cDNA. Cells, other than controls (C), were then treated with 25 mg/ml peptone (PEP), 1 μM LPA, or 1 μM FPP. The fold induction is the ratio of the value from each sample divided by the value from the control (no treatment), which was arbitrarily designated as 1. Bars are means ± SD (n = 3). *P < 0.05 relative to bar 1 of the corresponding color.