Fig. 1.

Levels of caveolin 1 (Cav1) and Src proteins and Cav1-Src interactions in intestinal epithelial cells (IECs). A: representative immunoblots of Cav1 and Src in IEC-6 cells and differentiated IEC-Cdx2L1 cells. Levels of total Cav1 and Src were examined by Western blot analysis, and actin immunoblotting was performed as an internal control for equal loading. B: levels of Cav1 and Src in materials immunoprecipitated (IP) by the anti-Src antibody (Ab) in cells described in A. After whole cell lysates (500 μg) were immunoprecipitated by the specific antibody against Src, precipitates were separated by SDS-PAGE. Levels of Cav1 and Src were measured using Western blot analysis with specific antibodies. IgG heavy chain is presented to show equal loading. Blots represent similar results from 3 separate experiments.