Fig. 5.

Effect of Src silencing by small interfering RNA (siRNA) targeting the Src mRNA coding region (siSrc) on levels of Src and Cav1, Cav1-Src interactions, and cell migration in differentiated IEC-Cdx2L1 cells. A: representative immunoblots of Src and Cav1. Cells were transfected with control siRNA (C-siRNA) or siSrc by Lipofectamine 2000, and whole cell lysates were harvested 48 h thereafter. Levels of Src and Cav1 proteins were analyzed by Western immunoblotting, and actin immunoblotting was performed as an internal control for equal loading. B: levels of Src, pCav1, and total Cav1 proteins in materials immunoprecipitated by the anti-Cav1 Ab from samples described in A. C: summary of cell migration data 6 h after wounding. Values are means ± SE from 6 dishes. *P < 0.05 compared with cells transfected with C-siRNA.