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Effect of NFs on cell proliferation and apoptosis. Cell proliferation and apoptosis are measured using Ki-67 and caspase-3 staining. Cell proliferation is significantly increased in WT cells seeded on NF compared with Matrigel after strain application (P < 0.05). No differences are observed in HG and db groups (top). The percentages of apoptotic cells are significantly reduced in WT and db cells when cultured on NFs compared with Matrigel controls (bottom).
