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. 2014 Jan 29;306(8):C745–C752. doi: 10.1152/ajpcell.00313.2013

Fig. 5.

Fig. 5.

Effect of IH on junction proteins in human lung microvascular endothelial cells A: immunofluorescence analysis of the distribution of zona occludens-1 (ZO-1) and vascular endothelial (VE)-cadherins in control (left), IH (left middle), 1 h post-IH (right middle), and IH + MnTMPyP-treated cells (right). Nuclei were stained with DAPI. The yellow arrows indicate sites of membrane ruffling as well as cytoplasmic redistribution of VE-cadherins and ZO-1. Bar in the control at top = 20 μm. B and C: quantitative analyses of the distribution of ZO-1 (B) and VE-cadherins (C) are shown. Control (n = 17 cells), IH (n = 16 cells), 1 h post-IH (n = 15), and IH + MnTMPyP (n = 16 cells). Data (means ± SE) are expressed as percentage of control. **P < 0.001, compared with vehicle-treated control.