Fig. 8.

MAP kinase inhibitors prevent IH-induced reorganization of cytoskeleton and cell junction proteins in human lung microvascular endothelial cells. A: immunofluorescence analysis of the distribution of actin, cortactin, ZO-1, and VE-cadherins in cells exposed to IH 30 cycles treated with either vehicle (left); 30 μM SP600125, an ERK inhibitor (middle); or 30 μM PD98059, a JNK inhibitor (right). Nuclei were stained with DAPI. Bar at top left = 20 μm. B–D: quantitative analyses of cortactin (B), ZO-1 (C), and VE-cadherins (D) distribution in IH-exposed cells are shown. V: vehicle treated (n = 18 cells each); SP: SP600125 treated (n = 17 cells each); PD: PD98059 treated (n = 15 cells each) cells. Data (means ± SE) are expressed as percentage of control. **P < 0.001.