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. 2014 Jan 31;306(8):L797–L807. doi: 10.1152/ajplung.00347.2013

Fig. 1.

Fig. 1.

Interleukin-1β (IL-1β) increases MUC5AC mRNA abundance in lung epithelial cells in a concentration- and time-dependent manner. Differentiated human bronchial epithelial (HBE) (A) or A549 (B) cells were exposed to IL-1β (1, 10, or 100 ng/ml) or vehicle for 24 h. Differentiated HBE (C) or A549 (D) cells were incubated with 10 ng/ml of IL-1β or vehicle for 0, 1, 2, 4, 6, or 24 h. MUC5AC mRNA levels were quantified by qPCR and normalized to actin mRNA levels. Fold changes were determined by comparing levels of IL-1β-exposed cells to control levels. Experiments were carried out in triplicate using HBE cells from two individuals. Data are expressed as means ± SE. Statistically significant differences were analyzed by one-way ANOVA (Bonferroni method). *P < 0.05; ***P < 0.001.