Fig. 13.

Dermal fibrosis and lipoatrophy in the pump model. Mice were treated with pumps containing saline vehicle or bleomycin and then killed on day 35. Skin above the pumps was harvested, and paraffin sections were cut and stained with H&E or Masson's trichrome. A: histological staining shows increased dermal thickness induced by bleomycin and an almost complete loss of the adipose cell layer. B: quantification of A for dermal thickness based on measurements of 5 sites/field imaged at ×100 magnification, 6 fields/section from sections from 6 mice in each category. Numbers presented are the averages ± SE. ***P < 0.001.