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. 2014 Feb 18;306(8):E945–E964. doi: 10.1152/ajpendo.00473.2013

Fig. 5.

Fig. 5.

Immortalized cell lines from different adipose tissue depots. A: Oil Red O staining of differentiated cells on days 8–10 of differentiation. B: mRNA expression of UCP1, PPARγ, and preadipocyte factor (Pref-1; n = 3) and a representative example of Western blot for UCP1, CIDEA, and aP2. C: mRNA expression in cells from different immortalized cell lines on days 0, 2, and 8 of differentiation. Data are expressed as fold induction compared with BAT cells on day 0; n = 3. D: mRNA expression in differentiated cells from the different immortalized cell lines that had been treated for 5 h with β3-receptor agonist CL-316,243 (10 μM) or vehicle control (Ctrl). Data are expressed as fold induction compared with nonstimulated BAT cells; n = 3. For all of the mRNA data, bars represent means ± SE, and significant differences are shown. *P < 0.05; **P < 0.005; ***P < 0.001. PGC-1α, PPARγ coactivator-1α.