Fig. 6.

Transcriptional regulation of HGF by NF-κB and peroxisome proliferator-activated receptor-γ (PPARγ). A: NF-κB p65 inhibition of the HGF gene promoter. The test was conducted using the Hgf-luc reporter in HEK 293 cells. The promoter was induced by PPARγ plus retinoid X receptor (RXR) α. p65 was used to enhance NF-κB activity in cotransfection. B: RNA polymerase II (Pol II) signal in the Hgf promoter. Chromatin immunoprecipitation (ChIP) assay was conducted at the PPARγ response element. MEFs were treated with TNF-α (20 ng/ml) for 30 min. C: HDAC3 in NF-κB activity. Inhibition of PPARγ activity by p65 was examined in cotransfection. The Hgf-luc reporter was used to determine the inhibition in cotransfection of HEK 293 cells. Vector based-Hdac3 RNAi (HDAC3i) was used in cotransfection. The results are expressed as means ± SE (n = 3). Compared with control or WT: **P < 0.01 and ***P < 0.001.