Fig. 3.

Prostaglandin E₂ (PGE₂) release from primary WT and BERKO calvarial osteoblasts. PGE₂ release normalized to total protein in WT and BERKO cells exposed to S or 4 h of F in the absence (VEH) and presence of E₂. Each group was comprised of 4–7 replicates. Bars represent means ± SE. Genotype (P < 0.05), F (P < 0.0001), E₂ (P < 0.01), and genotype × F (P < 0.05) had significant main factor effects by a 3-way ANOVA. ^aP < 0.01 vs. WT + VEH S; ^bP < 0.0001 vs. BERKO + VEH S; ^cP < 0.001 vs. WT + E₂ S; ^dP < 0.0001 vs. BERKO + E₂; ^eP < 0.0001 vs. all other S and F groups by Fisher's PLSD post hoc analysis.