Fig. 5.

Relative (firefly/Renilla) luciferase activity in WT and BERKO primary calvarial cells. Cells were cotransfected with an estrogen response element luciferase (firefly) reporter plasmid and a control thymidine kinase-driven Renilla luciferase vector plasmid, treated with VEH or E₂, and subjected to static conditions or 12 h of OFF. Each group was comprised of 3–4 replicates. Bars represent means ± SE. Genotype (P < 0.001) and OFF (P < 0.05) had significant main factor effects by a 3-way ANOVA. E₂ treatment had no significant effect. ^aP < 0.05 vs. WT + VEH S; ^bP < 0.01 vs. WT + VEH F; ^cP < 0.05 vs. WT + E₂ S; ^dP < 0.001 vs. WT + E₂ F; ^eP < 0.05 vs. BERKO + E₂ S by Fisher's PLSD post hoc analysis.