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Increased OPN expression in the heart activates JNKs, increases Bax expression, and decreases Bcl2/Bax ratio. Left ventricular lysates were analyzed by Western using anti-p-JNKs (A), anti-Bax (B), and anti-Bcl2 (C) antibodies. Protein loading was normalized using total JNKs or GAPDH immunostaining. Bottom: mean data normalized with JNK or GAPDH. *P < 0.05 vs. sham; n = 3 to 4.
