Figure 4.

Concentration-Dependent Assembly of Mto1/2[bonsai] and Mto2 into Higher-Order Complexes In Vitro

(A) Sedimentation of Mto1[9A1-bonsai]-GFP and Mto2-SZZ (or Mto2-S) in cell lysates, after Mto2-based partial purification from lysates, and after concentration of the partially purified material, as indicated. Quantitative western blotting from 2.5 hr isokinetic glycerol density-gradient centrifugation is shown. Solid triangles indicate measured S values; open triangles indicate extrapolated S values (see Figure S3H).

(B) Samples as in (A), but centrifuged for only 45 min.

(C) Sedimentation of Mto2-SZZ in mto1Δ cell lysates, Mto2-S after partial purification from mto1Δ lysates, and Mto2-S after concentration of the partially purified material from mto1Δ lysates. Centrifugation and quantification were as in (A).

(D) Samples as in (C), but centrifuged for 45 min.