Fig. 3.

Overview of HDX experiments. Each box reflects one peptide and contains five different colors for the incubation times of 15, 60, 300, 1200 and 3600 s (bottom up), respectively. Individual colors correspond to the difference in relative deuteration (ΔD_rel) of two compared states according to the legend on the left. MS/MS confirmed peptides are marked with diamonds and arrowheads at box termini indicate continuation of the peptide in the previous or following line. Secondary structure elements are taken from DSSP (Define Secondary Structure of Proteins) analysis of BlrP1, PDB ID: 3GG0 [10]. Numbering corresponds to the wild-type protein (UNP ID: A6T8V8) and negative values originate from the purification tag [10]. Secondary structure elements are indicated above the sequence and their labels are colored purple and green for BLUF and EAL domains, respectively. Zooming in on the electronic version allows viewing full details of all comparisons. (a) Cc_d–Mg_d. (b) Cc_l–Cc_d. (c) Mg_l–Mg_d. (d) Cc_l–Mg_l. Animations of the time course of ΔD_rel for all four comparisons with corresponding coloration of the BlrP1 crystal structure are shown in Movies S1–S4.