Fig. 1.

CNB-001 reconciles palmitic acid-induced insulin resistance in cultured myotubes. (A) Cytotoxicity of CNB-001 was determined in quiescent C2C12 myotubes using MTT test (n = 3). Myotubes with 0.5% DMSO in cell culture media were used as a vehicle control. Data presented as a percentage of untreated control absorbance. TC₅₀ of 15.3 μM was extrapolated from the graph (B) [³H]2-Deoxyglucose-uptake assay (n = 5) in C2C12 myotubes, which were incubated with PA (0.4 mM) in the presence or absence of CNB-001 for 12 hours, and stimulated with insulin for 30 minutes (50 nM, 30 minutes). *P < 0.05 compared with corresponding no insulin conditions, †P < 0.05 compared with insulin stimulated cells, ‡P < 0.05 compared with PA + insulin treated cells.