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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Chem Biol. 2014 Feb 27;21(3):338–344. doi: 10.1016/j.chembiol.2014.01.009

Figure 3. In vitro phototherapy using IR700DX-mbc94.

Figure 3

(a) Comparison of phototherapy effect on CB2-mid DBT cells among four groups: IR700DX-mbc94 (−) & irradiation (−); IR700DX-mbc94 (−) & irradiation (+); IR700DX-mbc94 (+) & irradiation (−); and IR700DX-mbc94 (+) & irradiation (+). (b) Phototherapy effect on CB2-mid DBT cells incubated with 5 μM of IR700DX-mbc94 with or without wash. (c) Quantification of unbound (photosensitizer that was washed out) and bound (remaining photosensitizer after washing) IR700DX-mbc94 using fluorescence. (d) Phototherapy effect compared between CB2-mid DBT cells treated with IR700DX-mbc94 (5 μM) and free IR700DX dye (5 μM). (e) Comparison of cell death caused by IR700DX-mbc94 exposed to light irradiation between CB2-mid DBT and WT-DBT cells (**p<0.01). (f) Effect of DMTU (1 mM) inhibition on cell death caused by IR700DX-mbc94 phototherapy. (g) Effect of sodium azide (50 mM) inhibition on cell death caused by IR700DX-mbc94 phototherapy. Each data point represents the mean ± SD based on triplicate samples.