Immunocytochemical localization of wild type (A) and mutant (E) PRCD in COS-7 cells. No co-localization was observed with phalloidin labeled actin (B, F), or nuclei stained with DAPI (C, G) in the merged images (D, H). Localization of the His tag was present in a diffuse web-like distribution in most cells transfected with the wild type construct (A, D), whereas labeling was generally concentrated in vesicular profiles in the cytoplasm in cells transfected with the mutant construct (E, H). Scale bar: 25µm.