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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Virology. 2014 Feb 7;0:191–201. doi: 10.1016/j.virol.2014.01.014

Fig. 1. CD4 T cells from one healthy adult recognize peptide pools and individual RV peptides.

Fig. 1

PBMC from HA-06 were stimulated with peptide pools (A), or individual peptides (B), at a concentration of 1 µg/ml or with different doses (C) during 10h at 37°C and for the last 5h, 1 µg/ml of brefeldin A was added. The frequencies of T cells producing IL-2 and IFN-γ were evaluated by intracellular cytokine staining. SEB was used as a positive control. Responses were considered positive if the number of IL-2 or IFN-γ-producing CD4 T cells was at least twice that of the DMSO control and above 0.02% (dashed lines). In the experiments shown T cells did not produce IFN-γ.