Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Virology. 2014 Feb 7;0:191–201. doi: 10.1016/j.virol.2014.01.014

Fig. 4. Phenotype and expression of homing receptors of CD4 tetramer+ T cells of healthy adults.

Fig. 4

Fresh PBMC were obtained from HLA-DRB1*0101 healthy adults and stained with the Flu-tetramer (left panels), the TRF-tetramer (center panels) or the VP6-7-tetramer (right panels) and antibodies against differentiation markers and intestinal homing receptors. After gating on live CD3 T cells, total (A) or tetramer+ (B) CD4 T cells (top panels) were analyzed to identify antigen experienced T cells CD62L−CD45RA+/− and CD62L+CD45RA− (middle panels). The expression of α4β7 and CCR9 intestinal homing receptors was evaluated in antigen experienced cells (lower panels). Numbers in parenthesis in the lower row dot plots indicate number of events. Results from the eight volunteers for antigen experienced T cells VP6-7-tetramer+ and Flu-tetramer+ cells (C) and the expression of intestinal homing receptors (D) are summarized. Statistically significant differences determined with the Wilcoxon test are shown.