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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biol Cell. 2014 Feb 25;106(4):126–138. doi: 10.1111/boc.201400003

Figure 4. Loss of Set1-dependent methylation does not affect the recruitment of U1 snRNP to CBC-associated mRNPs.

Figure 4

Nuclear mRNPs were purified from WT or set1Δ cells expressing Cbc2-TAP and Prp42-HA. Co-purifying proteins were detected by Western Blot with anti-HA or anti-Mex67 antibodies (left panel). The ratio of mRNP-associated proteins relative to the WT cells and to the immuno-purified Cbc2-TAP was determined from at least 3 independent experiments (mean ± SD) (right panel). Significance of the differences observed between both strains was evaluated using Student t-test (**P 0.001–0.01).