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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biol Cell. 2014 Feb 25;106(4):126–138. doi: 10.1111/boc.201400003

Figure 6. H2B ubiquitylation promotes to RNAPII Ser2 phosphorylation and Npl3 recruitment on a subset of intron-containing genes.

Figure 6

(A) Phosphorylation of RNAPII Ser2 on indicated intron-containing genes was analyzed by ChIP assay in WT and htb1K123R cells using antibodies to RNAPII and phosphoSer2 specific antibodies. The RNAPII Ser2P/RNAPII ratio is shown. (B) Recruitment of Npl3 on indicated intron-containing genes was analyzed by ChIP assay in WT and htb1K123R cells using anti Npl3 antibodies and normalized to the intergenic region. Significance of the differences observed between both strains was evaluated using Student t-test (*P 0.01– 0.05; **P 0.001–0.01).