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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Exp Dermatol. 2014 Mar;23(3):189–194. doi: 10.1111/exd.12346

Figure 1.

Figure 1

Dynamic infiltration of CD4 and CD8 lymphocytes in skin wounds and their expression of cytokines. A) Numbers of positively stained CD4 and CD8 cells in the wounds and wound margins. N=5 in each group. # p<0.01 compared to CD8+ cells, two-way ANOVA followed by a Tukey’s multiple comparisons test was used. B&C) Flow cytometry analyses of CD4+ (gate R4) and CD8+ (gate R3) cells in spleen and day 7 wounds, respectively. Dead cells were gated out using 7-AAD staining. D&E) Representative flow cytometry demonstrating CD4+/CD11a+ or CD8+/CD11a+ (gate 12) populations in wounds. F) Relative mRNA expression of IL-10, IL-17, IFN-γ and TGF-β1 in CD4 and CD8 cells isolated from day 7 wounds. N=3 in each group. * p<0.05, # p<0.01 compared to spleen samples by t test. ND: not detectable. Error bars = standard deviation.