Fig. 4.

DGKη does not affect intracellular calcium stores. Calcium mobilization in HEK293 cells expressing (black) RFP alone or (red) RFP-DGKη after (A) stimulation with 1 µM thapsigargin or (B) stimulation with 10 µM carbachol in the absence of extracellular calcium. AUC measurements extended 8 minutes (A) or 4 minutes (B) after stimulation. Data are the average of two (A) or five (B) independent experiments; n = 69–190 cells per condition. ***P < 0.001 when compared with RFP alone (unpaired t test). All data, including calcium traces, are presented as means ± S.E.