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DGKη does not translocate within cells after GPCR stimulation. Confocal images of live HEK293 cells expressing RFP-DGKη after stimulation with (A) 10 µM carbachol or (B) 500 mM sorbitol for the indicated times. Cell culture and imaging conditions were identical to those used in calcium mobilization experiments. (A) Cells from independent experiments are shown in each row.
